Conjugation
Part:BBa_K1510831:Design
Designed by: Kao Jung Chang Group: iGEM14_NYMU-Taipei (2014-10-09)
Fragrance generator part2: alcohol acetyltransferase I and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 117
Illegal BamHI site found at 1444 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 544
Illegal SapI.rc site found at 1552
Design Notes
To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.
Source
acetyltransferase I derived from ATF1 from Saccharomyces cerevisiaeand BBa_B0034 from 2014 igem distribution.