Conjugation

Part:BBa_K1510831:Design

Designed by: Kao Jung Chang   Group: iGEM14_NYMU-Taipei   (2014-10-09)


Fragrance generator part2: alcohol acetyltransferase I and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
    Illegal BamHI site found at 1444
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544
    Illegal SapI.rc site found at 1552


Design Notes

To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.



Source

acetyltransferase I derived from ATF1 from Saccharomyces cerevisiaeand BBa_B0034 from 2014 igem distribution.

References